Waterstones Wimbledon will close next month, with staff redeployed to other branches.
The shop's last day of trading will be 15th August after the premises was repossessed by its landlord for redevelopment.
A spokesperson for Waterstones said the company "much regrets" the shop's closure and as yet "Waterstones has been unable to secure a suitable alternative site in Wimbledon."
The store has six members of staff who will be offered redeployment to other shops in the Waterstones estate.
The Bookseller also understands that WH Smith in Wimbledon is in negotiation with the landlord over its future tenancy.